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  • Twenty-five 2020/05

    Roof placement The key technology of Visium space transcriptome: frozen section preparation

    In the previous few years, Xiaobian has briefly introduced the principles and procedures of the Visium space transcriptome. (space transcriptome) have you understood the principle and sample preparation of the first bomb? As well as the experimental design and research ideas of the high score articles, we can see that the preparation of frozen slices is the focus of the whole space transcriptome experiment, which determines the quality of the sequencing data. In order to get a slice that can indicate the true expression level in the tissue, the experimental process needs to be completed in a low temperature environment to ensure the stability of RNA in the tissue. This also greatly tests the proficiency and professionalism of the experimenters. Now let's talk about the frozen sections. Most tissue samples far exceed the area of the chip capture area (6.5mmX6.5mm). Therefore, the design of cutting area and cutting section is very important for maximizing sample utilization and minimizing cost. 2. Most of the tissue samples are far more than the slices.

    Eleven One hundred and thirty-four Learn more.
  • Fourteen 2020/04

    Roof placement [space transcriptome] one of the second shells will help you understand the ideas and applications of space transcriptome research.

    In the last issue, we introduced the principles and procedures of space transcriptome sequencing, especially in the process of sample preparation. (space transcriptome) do you understand the principle of first bomb and sample preparation? Today, Xiaobian combines some specific cases to share some analysis ideas of current space transcriptome. First of all, I want to share with you an article published in NatureCommunication 18 years ago in June. This study analyzed the characterization of spatial differential genes and pathways by using ST sequencing. Publication time: 2018.06 published journal: NatureCommunication impact factor: 11.878 research direction: cancer prostate cancer research strategy: radical resection of prostate cancer tissue, according to pathological annotation made 12 tissue sections (location as shown above), a total of 5910 regions were analyzed, and the use of deconvolution method to analyze transcriptome data.

    Twelve Two hundred and eighty-eight Learn more.
  • 08 2020/04

    Roof placement Do you know the principles and sample preparation of the first bomb?

    In fact, the concept of space transcriptome has existed since the 2016, and the current transcriptome technology has Slide-seq, LCM-seq, seqFISH, MERFISH, Liversinglecellzonation and Tomo-seq. However, these methods are complex in operation, inaccurate in detection accuracy, and limited in gene and cell detection limits. The improved Visium space transcript based on SpatialTranscriptomics upgrade is the only commercially accurate transcriptome sequencing protocol with high accuracy and high throughput. The workflow is simple, and the construction from tissue slicing to library construction takes only one day, which greatly saves time and cost. The essence of this method is to capture the expression pattern and spatial distribution of RNA in different regions of frozen tissue slices by high-throughput in situ probe capture by spatial barcode. When tissue frozen sections are attached with spa

    Eleven Three hundred and nineteen Learn more.
  • Thirty 2020/03

    Roof placement New perspective of Nature review|| reveals cancer -- starting from CAF

    In recent years, the study of Thetumormicroenvironment (TME) has been focused on a more precise and detailed research level as a microscope. It plays a decisive role in revealing the mechanisms of tumor growth and metastasis, and plays an important role in the diagnosis, prevention and treatment of tumors. In TME, tumor associated fibroblasts (cancer-associatedfibroblasts, CAFs) play an important role in the formation and development of TME. CAFs can remold extracellularmatrix (ECM), secrete growth factor (growthfactor), affect angiogenesis (angiogenesis), mediate immune system (immunesystem), and so on. It has dual functions of promoting tumor and inhibiting tumor, research on tumor mechanism and tumor drug therapy.

    Eleven Three hundred and sixty-seven Learn more.
  • Twenty-four 2020/03

    Roof placement Single cell literature inventory February single cell high score literature summary

    In the current issue, Xiaobian will take a look at the high score articles related to single cell transcriptome sequencing in February. In ~2, 21 high score articles (IF>20) were colleced in the month. Among them, there were 6 articles on single cell map, including the first human thymocyte map, primate aging ovarian cell map, and single chip for mouse intestine, skin, suprachiasmatic nucleus and endothelial cells. The cell transcriptional map was plotted. There are 6 articles about growth and development, including stem cell differentiation, gastrula, and organ like; tumor related literature 3, immune related literature 4, neuroscience 2. (scanning the two-dimensional code, getting all the articles ~) below, Xiaobian will briefly introduce 7 CNS documents: 1. Topic: MAFG-drivenastrocytespromoteCNSinflammationDOI: 10.1038/s41586-020-1999-0: Journal: Nature influence

    Thirteen Two hundred and fifty-nine Learn more.
  • Sixteen 2020/03

    Roof placement Technology preemptive look at new space transcriptome sequencing data analysis strategy

    In the previous few years, Xiaobian has shared the articles of single cell and space transcriptome applied to bone marrow hematopoietic microenvironment. (interested little partners can make up lessons.) in this issue, Xiaobian will focus on the analysis strategy of the space transcriptome data of the frozen organisms for human samples. The joint analysis process of single cell transcriptome sequencing and space transcriptome sequencing is also in the final stage of optimization. We are looking forward to seeing you. First of all, let's share with you a high score article published in NatureBiotechnology in January 13, 2020: Integratingmicroarray-basedspatialtranomicsandsingle-cellRNA-seqrevealstissuearchitectureinpancreaticductaladeno

    Fifteen Four hundred and thirty-seven Learn more.
  • Thirteen 2020/03

    Roof placement 10th anniversary great benefit, ice and methylation sequencing to help new heights of research.

    10th anniversary ex gratia first shot: CpG Island precise methylation sequencing one-stop service is now only 2999 yuan / case (yes, including the whole sequencing process and analysis process), low breakdown. Let you spend the least money to develop the best quality sequencing projects and experience the best service. CpG Island precise methylation sequencing focuses on human and mouse databases, with high accuracy, deep sequencing and high capture efficiency, which can serve better for medical biological research. If you are interested in doing related research or do not know the specific operation, you can communicate with us at any time by phone or email. We are always ready to provide you with a complete methylation research solution. Scan the bottom two dimensional code, pay attention to the public number "strong ice creatures", restore the "methylation activity" backstage, and get the exact methylation sequencing research plan of CpG island.

    Thirteen Two hundred and sixty-eight Learn more.
  • Ten 2020/03

    Roof placement Single nuclear transcriptome sequencing (snRNA-Seq) reveals differences between humans and mice in Alzheimer's disease

    A few days ago, Xiaobian shared with you the research of single cell TCR sequencing technology in Alzheimerdisease (AD). Today, strong Xiaobing will bring about the research of Single-nucleusRNAsequence (snRNA-Seq) in AD. 1. Single cell nuclear transcriptome sequencing (snRNA-Seq) technology has been developing vigorously with the development of single cell transcriptome sequencing (scRNA-Seq) technology in many aspects, such as embryo development, tumor cell heterogeneity, immune cell transformation and so on. However, because of the different cell morphology and the difference of cell sensitivity in single cell preparation, scRNA-Seq may be in data analysis. The proportion of cell types was distorted, and some cell types were lost. In order to overcome the limitations of single cell sequencing technology, single nuclear transcriptome sequencing (snRNA-Seq) technology should be transported.

    Eleven Five hundred Learn more.
  • 06 2020/03

    Roof placement Inventory of single cell high score in January

    In recent years, Xiaobian shared a number of high score literature related to single cell transcriptome sequencing. Many small partners were not interested in it. Then, Xiaobian summarized the scRNA-Seq related high score articles published in January 2020, and published the journal, research direction and analysis platform. The specific information is as follows: 1, topic: Invitrocharacterizationofthehumansegmentati OnclockDOI: 10.1038/s41586-019-1885-9 published: Nature impact factor: 44.07 date of publication: 20200108 research direction: development spine summary: spinal segmental tissue was established at the early stage of embryonic development, when paired segments were rhythmically generated by the anterior segment mesoderm (PSM). The rhythm of the formation of the body is controlled by a molecular oscillator called a segmented clock. Although this kind of vibration

    Thirteen Four hundred and twenty Learn more.
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